![](https://parts.igem.org/images/partbypart/icon_dna.png)
DNA
Part:BBa_K4373003:Design
Designed by: Mandy Hobusch, Leon Eisentraut Group: iGEM22_Lund (2022-09-24)
C-P-Lyase encoding sequence
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1627
Illegal EcoRI site found at 1969
Illegal PstI site found at 1654
Illegal PstI site found at 3061
Illegal PstI site found at 3360
Illegal PstI site found at 3833 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1627
Illegal EcoRI site found at 1969
Illegal PstI site found at 1654
Illegal PstI site found at 3061
Illegal PstI site found at 3360
Illegal PstI site found at 3833 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1627
Illegal EcoRI site found at 1969
Illegal BglII site found at 837
Illegal BglII site found at 1605
Illegal BamHI site found at 1461
Illegal XhoI site found at 1056 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1627
Illegal EcoRI site found at 1969
Illegal PstI site found at 1654
Illegal PstI site found at 3061
Illegal PstI site found at 3360
Illegal PstI site found at 3833 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1627
Illegal EcoRI site found at 1969
Illegal PstI site found at 1654
Illegal PstI site found at 3061
Illegal PstI site found at 3360
Illegal PstI site found at 3833
Illegal NgoMIV site found at 2143
Illegal NgoMIV site found at 3018
Illegal NgoMIV site found at 3458
Illegal AgeI site found at 662
Illegal AgeI site found at 1121
Illegal AgeI site found at 1497 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was optimized for codon usage in Bacillus subtilis. Nucleotides forming restriction sites HindIII, NheI and PfI23II inside the gene were changed according to the codon triplet.
Source
Gene sequence for this part was obtained from [3].
References
[1] Lopes Catão, A. J., & López-Castillo, A. (2018). On the degradation pathway of glyphosate and glycine. Environmental Science: Processes & Impacts, 20(8), 1148–1157. https://doi.org/10.1039/c8em00119g[2] Quinn, J. P., & Dick, J. E. (1994, October 24). Glyphosate-degrading isolates from environmental samples: occurrence and pathways of degradatio. Applied Microbiology Biotechnology, 43, 545–550.
[3] McLean, P.A., Liu C.M, Sookdeo, C.C., Cannon, F.C.(1992).Characterization of a gene cluster involved in utilization of glyphosate and other phosphonates in Rhizobium meliloti. NCBI Genebank https://www.ncbi.nlm.nih.gov/nuccore/M96263.1/