DNA

Part:BBa_K4373003:Design

Designed by: Mandy Hobusch, Leon Eisentraut   Group: iGEM22_Lund   (2022-09-24)


C-P-Lyase encoding sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1627
    Illegal EcoRI site found at 1969
    Illegal PstI site found at 1654
    Illegal PstI site found at 3061
    Illegal PstI site found at 3360
    Illegal PstI site found at 3833
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1627
    Illegal EcoRI site found at 1969
    Illegal PstI site found at 1654
    Illegal PstI site found at 3061
    Illegal PstI site found at 3360
    Illegal PstI site found at 3833
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1627
    Illegal EcoRI site found at 1969
    Illegal BglII site found at 837
    Illegal BglII site found at 1605
    Illegal BamHI site found at 1461
    Illegal XhoI site found at 1056
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1627
    Illegal EcoRI site found at 1969
    Illegal PstI site found at 1654
    Illegal PstI site found at 3061
    Illegal PstI site found at 3360
    Illegal PstI site found at 3833
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1627
    Illegal EcoRI site found at 1969
    Illegal PstI site found at 1654
    Illegal PstI site found at 3061
    Illegal PstI site found at 3360
    Illegal PstI site found at 3833
    Illegal NgoMIV site found at 2143
    Illegal NgoMIV site found at 3018
    Illegal NgoMIV site found at 3458
    Illegal AgeI site found at 662
    Illegal AgeI site found at 1121
    Illegal AgeI site found at 1497
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was optimized for codon usage in Bacillus subtilis. Nucleotides forming restriction sites HindIII, NheI and PfI23II inside the gene were changed according to the codon triplet.

Source

Gene sequence for this part was obtained from [3].

References

[1] Lopes Catão, A. J., & López-Castillo, A. (2018). On the degradation pathway of glyphosate and glycine. Environmental Science: Processes & Impacts, 20(8), 1148–1157. https://doi.org/10.1039/c8em00119g

[2] Quinn, J. P., & Dick, J. E. (1994, October 24). Glyphosate-degrading isolates from environmental samples: occurrence and pathways of degradatio. Applied Microbiology Biotechnology, 43, 545–550.

[3] McLean, P.A., Liu C.M, Sookdeo, C.C., Cannon, F.C.(1992).Characterization of a gene cluster involved in utilization of glyphosate and other phosphonates in Rhizobium meliloti. NCBI Genebank https://www.ncbi.nlm.nih.gov/nuccore/M96263.1/